Laminin Fibrils in Newt Gastrulae Visualized by the Immunofluorescent Staining. (gastrulation/laminin/immunofluorescent staining/newt/amphibian)
نویسندگان
چکیده
منابع مشابه
Immunofluorescent Staining: the Fluorescent Antibody Method.
I. Principles of Tmmunofluorescent Staining.................................................. A. Fluorescent Antibody Labeling........................................................ B. Immunofluorescent Staining Procedures............................................... C. Controls.............................................................................. D. Nonspecific Staining................
متن کاملVisualization of numerical centrosomal abnormalities by immunofluorescent staining.
The presence of multiple centrosomes in tumor cells is associated with the formation of multipolar mitotic spindles and results in aneuploidy of both daughter cells. Centrosome amplification is a feature of all cancer cells. We have previously described centrosome amplification in abnormal B cells. Further studies of centrosome amplification in different stages of B lineage development could pr...
متن کاملDetection of Legionnaires disease bacteria by direct immunofluorescent staining.
Antisera and fluorescein isothiocyanate conjugates prepared for five strains of the Legionnaires bacteria were tested in both homologous and heterologous staining reactions with 10 isolates of the organism from patients in seven geographic areas. The strains were related but not identical as judged by the results of direct immunofluorescence staining. The conjugates were successfully used to de...
متن کاملImmunofluorescent studies of fibronectin and laminin in the human eye.
The topographic distribution of fibronectin and laminin in young and old human eyes was determined by indirect immunofluorescent techniques. These two glycoproteins may play a role in the attachment of the vitreous to the internal limiting membrane (ILM) and the internal limiting membrane to the Mueller cell processes. A double-laminated pattern of fluorescence for both glycoproteins was freque...
متن کاملA Study of Newt Mitotic Chromosomes by Negative Staining
A method is described for bursting single, selected mitotic cells on a fluid surface. Cells from cultures of newt heart tissue were burst on dilute solutions containing potassium and sodium with and without added calcium and also on dilute calcium chloride solution. The material was negatively stained with uranyl acetate or sometimes with ammonium molybdate or sodium phosphotungstate. The bodie...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Development, Growth and Differentiation
سال: 1985
ISSN: 0012-1592,1440-169X
DOI: 10.1111/j.1440-169x.1985.00639.x